Immunolyser Report — b2f02e49-d592-4c83-8b8c-2b095b2b8be3

Immunolyser Offline Report — Job ID: b2f02e49-d592-4c83-8b8c-2b095b2b8be3
All images and analysis data are embedded in this file. Charts require an internet connection to load the Plotly/UpSetJS libraries on first open; subsequent opens may work fully offline if your browser has cached those libraries.

Peptide Length Distribution

ⓘ Peptide length distribution of uploaded samples. Peptides labelled #CONTAM or #DECOY are filtered out. Length range: 5–30 residues.

Percentage

Absolute Values

Peptide Overlap

ⓘ UpSet plot of peptide overlap across samples after pre-processing. For MHC class I: 8–14-mers; class II: 12–20-mers.

Peptide Consensus Binding Motif Analysis

ⓘ Seq2Logo showing consensus binding motifs after pre-processing.

Liver

Sample size: 4834

Spleen

Sample size: 3912

Skin

Sample size: 5173

Peptide Sequence Clustering with Predicted MHC Allele Assignment

ⓘ Gibbs Clustering applied after removing contaminants, decoys, PTMs, and duplicates. Each logo shows the predicted source allotype and PCC value (MHC-TP).

Liver

Hepa_Set1_DDA

KLD bar chart

Sample size: 3626

Best match: H2Kk
PCC score: 0.42

Sample size: 4944

Best match: H2Kb
PCC score: 0.54

Sample size: 3101

Best match: H2Db
PCC score: 0.6

KLD bar chart

Sample size: 10460

Best match: Allele not predicted
PCC score: Score not calculated

KLD bar chart

Sample size: 6920

Best match: H2Kb
PCC score: 0.58

Sample size: 4203

Best match: H2Kk
PCC score: 0.36

KLD bar chart

Sample size: 3626

Best match: H2Kk
PCC score: 0.42

Sample size: 4944

Best match: H2Kb
PCC score: 0.54

Sample size: 3101

Best match: H2Db
PCC score: 0.6

KLD bar chart

Sample size: 2038

Best match: H2Db
PCC score: 0.73

Sample size: 3472

Best match: H2Kk
PCC score: 0.43

Sample size: 3857

Best match: H2Kb
PCC score: 0.51

Sample size: 2525

Best match: H2Kd
PCC score: 0.27

KLD bar chart

Sample size: 1842

Best match: H2Db
PCC score: 0.71

Sample size: 2180

Best match: H2Kb
PCC score: 0.59

Sample size: 1748

Best match: H2Kd
PCC score: 0.27

Sample size: 2826

Best match: H2Kb
PCC score: 0.37

Sample size: 3348

Best match: H2Kk
PCC score: 0.39

KLD bar chart

Sample size: 3295

Best match: H2Kk
PCC score: 0.36

Sample size: 2771

Best match: H2Kb
PCC score: 0.61

Sample size: 1560

Best match: H2Db
PCC score: 0.74

Sample size: 1692

Best match: H2Kd
PCC score: 0.21

Sample size: 1192

Best match: H2Qa2
PCC score: 0.27

Sample size: 1541

Best match: H2Kd
PCC score: 0.23

Skin

Skin_Set1_DDA

KLD bar chart

Sample size: 6433

Best match: H2Kb
PCC score: 0.54

Sample size: 3973

Best match: H2Kk
PCC score: 0.4

KLD bar chart

Sample size: 9789

Best match: Allele not predicted
PCC score: Score not calculated

KLD bar chart

Sample size: 6433

Best match: H2Kb
PCC score: 0.54

Sample size: 3973

Best match: H2Kk
PCC score: 0.4

KLD bar chart

Sample size: 2824

Best match: H2Kd
PCC score: 0.33

Sample size: 4493

Best match: H2Kb
PCC score: 0.55

Sample size: 3664

Best match: H2Kk
PCC score: 0.38

KLD bar chart

Sample size: 3030

Best match: H2Dd
PCC score: 0.54

Sample size: 2907

Best match: H2Kd
PCC score: 0.39

Sample size: 1704

Best match: H2Kd
PCC score: 0.26

Sample size: 3510

Best match: H2Kk
PCC score: 0.42

KLD bar chart

Sample size: 1337

Best match: H2Qa2
PCC score: 0.27

Sample size: 1822

Best match: H2Kd
PCC score: 0.24

Sample size: 1986

Best match: H2Kb
PCC score: 0.57

Sample size: 3455

Best match: H2Kk
PCC score: 0.4

Sample size: 2665

Best match: H2Kb
PCC score: 0.34

KLD bar chart

Sample size: 1562

Best match: H2Kb
PCC score: 0.48

Sample size: 1210

Best match: H2Kd
PCC score: 0.16

Sample size: 3261

Best match: H2Kk
PCC score: 0.41

Sample size: 1209

Best match: H2Kd
PCC score: 0.3

Sample size: 1664

Best match: H2Kd
PCC score: 0.22

Sample size: 2485

Best match: H2Kb
PCC score: 0.5

Spleen

Spleen_Set1_DDA

KLD bar chart

Sample size: 1412

Best match: H2Qa2
PCC score: 0.29

Sample size: 1402

Best match: H2Dq
PCC score: 0.27

Sample size: 2190

Best match: H2Kk
PCC score: 0.4

Sample size: 2642

Best match: H2Kb
PCC score: 0.51

Sample size: 1635

Best match: H2Kd
PCC score: 0.26

KLD bar chart

Sample size: 8094

Best match: H2Kb
PCC score: 0.28

KLD bar chart

Sample size: 5305

Best match: H2Kb
PCC score: 0.56

Sample size: 3334

Best match: H2Kk
PCC score: 0.41

KLD bar chart

Sample size: 2417

Best match: H2Kk
PCC score: 0.46

Sample size: 2738

Best match: H2Kd
PCC score: 0.34

Sample size: 3878

Best match: H2Kb
PCC score: 0.55

KLD bar chart

Sample size: 2281

Best match: H2Kq
PCC score: 0.37

Sample size: 1616

Best match: H2Qa2
PCC score: 0.26

Sample size: 3062

Best match: H2Kb
PCC score: 0.54

Sample size: 2205

Best match: H2Kd
PCC score: 0.33

KLD bar chart

Sample size: 1412

Best match: H2Qa2
PCC score: 0.29

Sample size: 1402

Best match: H2Dq
PCC score: 0.27

Sample size: 2190

Best match: H2Kk
PCC score: 0.4

Sample size: 2642

Best match: H2Kb
PCC score: 0.51

Sample size: 1635

Best match: H2Kd
PCC score: 0.26

KLD bar chart

Sample size: 995

Best match: H2Db
PCC score: 0.35

Sample size: 1347

Best match: H2Qa2
PCC score: 0.3

Sample size: 1537

Best match: H2Kb
PCC score: 0.57

Sample size: 1497

Best match: H2Kd
PCC score: 0.25

Sample size: 1858

Best match: H2Kb
PCC score: 0.35

Sample size: 2144

Best match: H2Kk
PCC score: 0.4

MHC-TP (MHC Typing Prediction)

ⓘ Predicted MHC I alleles based on peptide clustering (MHC-TP). Note: interactive HTML result files are not included in this export.

Liver

Liver — Hepa_Set1_DDA — clust_result — clust-search-result.html (open full report on server to view)

Spleen

Spleen — Spleen_Set1_DDA — clust_result — clust-search-result.html (open full report on server to view)

Skin

Skin — Skin_Set1_DDA — clust_result — clust-search-result.html (open full report on server to view)

Peptide-HLA/MHC Binding Affinity Prediction — Downloads

ⓘ Majority-voted binder CSV files for each sample and allele. Click a replicate name to download.

Liver

Allele	Majority Voted Binders
H-2Kb	Hepa_Set1_DDA
H-2Db	Hepa_Set1_DDA

Spleen

Allele	Majority Voted Binders
H-2Kb	Spleen_Set1_DDA
H-2Db	Spleen_Set1_DDA

Skin

Allele	Majority Voted Binders
H-2Kb	Skin_Set1_DDA
H-2Db	Skin_Set1_DDA

Peptide-HLA/MHC Binding Affinity Prediction

ⓘ UpSet plot showing predicted binder overlap across samples. Select an allele and tool using the controls below.

H-2Kb

Liver

Hepa_Set1_DDA

Spleen

Spleen_Set1_DDA

Skin

Skin_Set1_DDA

H-2Db

Liver

Hepa_Set1_DDA

Spleen

Spleen_Set1_DDA

Skin

Skin_Set1_DDA